Publications

 

Journal publications

 

 
40. C-terminal glycosylation of type IX secretion system cargo proteins in Prevotella intermedia with both short and long secretion signals.
Ye X, Bin Rustam N, Gorasia D, Reynolds E, Ghosal D, Veith P. Open Biol. 2025 Mar;15(3):240335. doi: 10.1098/rsob.240335. Epub 2025 Mar 26. PMID: 40132644
39. Mutations in Gordonia amarae mycolic acid biosynthetic pathway confer resistance to Patescibacteria parasite Mycosynbacter amalyticus.
Rose JJA, Johnson MD, Reyhani M, Batinovic S, Seviour RJ, Ghosal D, Petrovski S. Nat Commun. 2025 Mar 5;16(1):2202. doi: 10.1038/s41467-025-56933-4. PMID: 40038264
38. DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp.
Reva ON, La Cono V, Marturano L, Crisafi F, Smedile F, Mudaliyar M, Ghosal D, Selivanova EA, Ignatenko ME, Ferrer M, Fernandez-Lopez L, Krupovic M, Yakimov MM. iScience. 2025 Jan 4;28(2):111749. doi: 10.1016/j.isci.2025.111749. eCollection 2025 Feb 21. PMID: 39925428
 
37. Membrane association and polar localization of the Legionella pneumophila T4SS DotO ATPase mediated by two nonredundant receptors.
Vijayrajratnam S, Milek S, Maggi S, Ashen K, Ferrell M, Hasanovic A, Holgerson A, Kannaiah S, Singh M, Ghosal D, Jensen GJ, Vogel JP. Proc Natl Acad Sci U S A. 2024 Oct 8;121(41):e2401897121. doi: 10.1073/pnas.2401897121. Epub 2024 Oct 1. PMID: 39352935
36. Cell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system.
Johnson MD, Shepherd DC, Sakai HD, Mudaliyar M, Pandurangan AP, Short FL, Veith PD, Scott NE, Kurosawa N, Ghosal D. Nat Commun. 2024 Aug 16;15(1):7066. doi: 10.1038/s41467-024-51159-2. PMID: 39152123
35. Large attachment organelle mediates interaction between Nanobdellota archaeon YN1 and its host.
Johnson MD, Sakai HD, Paul B, Nunoura T, Dalvi S, Mudaliyar M, Shepherd DC, Shimizu M, Udupa S, Ohkuma M, Kurosawa N, Ghosal D. ISME J. 2024 Jan 8;18(1):wrae154. doi: 10.1093/ismejo/wrae154. PMID: 39113594
34. Co-clustering of EphB6 and ephrinB1 in trans restrains cancer cell invasion.
Liang LY, Geoghegan ND, Mlodzianoski M, Leis A, Whitehead LW, Surudoi MG, Young SN, Janes P, Shepherd D, Ghosal D, Rogers KL, Murphy JM, Lucet IS. Commun Biol. 2024 Apr 16;7(1):461. doi: 10.1038/s42003-024-06118-4. PMID: 38627519
33. Interplay of intracellular and trans-cellular DNA methylation in natural archaeal consortia.
Reva ON, La Cono V, Crisafi F, Smedile F, Mudaliyar M, Ghosal D, Giuliano L, Krupovic M, Yakimov MM. Environ Microbiol Rep. 2024 Apr;16(2):e13258. doi: 10.1111/1758-2229.13258. PMID: 38589217
32. Staphylococcus epidermidis bacteriocin A37 kills natural competitors with a unique mechanism of action.
Puls JS, Winnerling B, Power JJ, Krüger AM, Brajtenbach D, Johnson M, Bilici K, Camus L, Fließwasser T, Schneider T, Sahl HG, Ghosal D, Kubitscheck U, Heilbronner S, Grein F. ISME J. 2024 Jan 8;18(1):wrae044. doi: 10.1093/ismejo/wrae044. PMID: 38470311
31. Ultrastructural and glycoproteomic characterization of Prevotella intermedia: Insights into O-glycosylation and outer membrane vesicles.
Ye X, Paul B, Mo J, Reynolds EC, Ghosal D, Veith PD. Microbiologyopen. 2024 Apr;13(2):e1401. doi: 10.1002/mbo3.1401. PMID: 38409911
30. Structural Insights into SARS-CoV-2 Nonstructural Protein 1 Interaction with Human Cyclophilin and FKBP1 to Regulate Interferon Production.
Vankadari N, Ghosal D. J Phys Chem Lett. 2024 Feb 1;15(4):919-924. doi: 10.1021/acs.jpclett.3c02959. Epub 2024 Jan 19. PMID: 38241259
29. Morphological remodeling of Coxiella burnetii during its biphasic developmental cycle revealed by cryo-electron tomography.
Shepherd DC, Kaplan M, Vankadari N, Kim KW, Larson CL, Dutka P, Beare PA, Krzymowski E, Heinzen RA, Jensen GJ, Ghosal D. iScience. 2023 Jun 24;26(7):107210. doi: 10.1016/j.isci.2023.107210. eCollection 2023 Jul 21. PMID: 37485371
28. Structure and Function of the Dot/Icm T4SS.
Dutka P, Liu Y, Maggi S, Ghosal D, Wang J, Carter SD, Zhao W, Vijayrajratnam S, Vogel JP, Jensen GJ. bioRxiv [Preprint]. 2023 Mar 22:2023.03.22.533729. doi: 10.1101/2023.03.22.533729. PMID: 36993699
27. Cryo-EM structure of the Agrobacterium tumefaciens T4SS-associated T-pilus reveals stoichiometric protein-phospholipid assembly.
Kreida S, Narita A, Johnson MD, Tocheva EI, Das A, Ghosal D, Jensen GJ. Structure. 2023 Apr 6;31(4):385-394.e4. doi: 10.1016/j.str.2023.02.005. Epub 2023 Mar 3. PMID: 36870333
26. Manipulation of epithelial cell architecture by the bacterial pathogens Listeria and Shigella.
Hartland EL, Ghosal D, Giogha C. Curr Opin Cell Biol. 2022 Dec;79:102131. doi: 10.1016/j.ceb.2022.102131. Epub 2022 Oct 7. PMID: 36215855
25. Discovery of a Novel Inner Membrane-Associated Bacterial Structure Related to the Flagellar Type III Secretion System.
Kaplan M, Oikonomou CM, Wood CR, Chreifi G, Ghosal D, Dobro MJ, Yao Q, Pal RR, Baidya AK, Liu Y, Maggi S, McDowall AW, Ben-Yehuda S, Rosenshine I, Briegel A, Beeby M, Chang YW, Shaffer CL, Jensen GJ. J Bacteriol. 2022 Aug 16;204(8):e0014422. doi: 10.1128/jb.00144-22. Epub 2022 Jul 18. PMID: 35862756
24. Archaic chaperone-usher pili self-secrete into superelastic zigzag springs.
Pakharukova N, Malmi H, Tuittila M, Dahlberg T, Ghosal D, Chang YW, Myint SL, Paavilainen S, Knight SD, Lamminmäki U, Uhlin BE, Andersson M, Jensen G, Zavialov AV. Nature. 2022 Sep;609(7926):335-340. doi: 10.1038/s41586-022-05095-0. Epub 2022 Jul 19. PMID: 35853476
23. Structure of Human TMPRSS2 in Complex with SARS-CoV-2 Spike Glycoprotein and Implications for Potential Therapeutics.
Vankadari N, Ketavarapu V, Mitnala S, Vishnubotla R, Reddy DN, Ghosal D. J Phys Chem Lett. 2022 Jun 8:5324-5333. doi: 10.1021/acs.jpclett.2c00967. Online ahead of print. PMID: 35675654
22. Three-dimensional insights into human enveloped viruses in vitro and in situ. Vankadari N, Shepherd DC, Carter SD, Ghosal D. Biochem Soc Trans. 2022 Feb 28;50(1):95-105. doi: 10.1042/BST20210433. PMID: 35076655
 

21. From cells to atoms: Cryo-EM as an essential tool to
investigate pathogen biology, host-pathogen interaction, and drug discovery.Shepherd DC, Dalvi S, Ghosal D. Mol Microbiol. 2022 Mar;117(3):610-617. doi: 10.1111/mmi.14820. Epub 2021 Oct 23. PMID: 34592048

20. Vankadari N, Ketavarapu V, Mitnala S, Vishnubotla R, Reddy DN, Ghosal D (2022). Structure of Human TMPRSS2 in Complex with SARS-CoV-2 Spike Glycoprotein and Implications for Potential Therapeutics.  J Phys Chem Lett. Jun 8;13(23):5324-5333.

 

19.  Vankadari N, Shepherd DC, Carter SD, Ghosal D, Ghosal D (2022). Three-dimensional insights into human enveloped viruses in vitro and in situ. Biochem Soc Trans. Feb 28;50(1):95-105..

 

18. Shepherd D, Dalvi S, Ghosal D (2022). From cells to atoms: Cryo-EM as an essential tool to investigate pathogen biology, host-pathogen interaction, and drug discovery. Mol Microbiol, Mar;117(3):610-617.

17. Kaplan M, Nicolas WJ, Zhao W, Carter SD, Metskas LA, Chreifi G, Ghosal D, Jensen GJ. (2021). In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography. Methods Mol Biol, 2021;2215:83-111.

 

16. Nicolas WJ, Ghosal D, Tocheva EI, Meyerowitz EM, Jensen GJ (2020). Structure of the Bacterial Cellulose Ribbon and Its Assembly-Guiding Cytoskeleton by Electron Cryotomography. J Bacteriol, 11;203(3):e00371-20.

 

15. Ghosal D, Kim KW, Zheng H, Kaplan M, Truchan HK, Lopez AE, McIntire IE, Vogel JP, Cianciotto NP, Jensen GJ. (2019) In vivo structure of the Legionella type II secretion system by electron cryotomography. Nature Microbiology, Dec; 4(12):2101-2108.

14. Ghosal D, Jeong KC, Chang YW, Gyore J, Teng L, Gardner A, Vogel JP and Jensen GJ. (2019) Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS. Nature Microbiology, Jul; 4(7):1173-1182.

·     Reported in 1) Phys.Org 2) Caltech news 3) The Science Times 4) Eurekalert 5) Science Daily etc.

13. Kaplan M, Ghosal D, Subramanian P, Oikonomou C, Kjær A, Pirbadian S, Ortega DR, El-Naggar MY and Jensen GJ. (2019) The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type. eLife, 16;8. pii: e43487.

12. Ghosal D*, Kaplan M*, Chang YW, Jensen GJ. (2019) In Situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems Using Electron Cryotomography. Methods Mol Biol. 1921:249-265.

11. Kaplan M, Subramanian P, Ghosal D, Oikonomou C, Pirbadian S, Starwalt‐Lee R, Mageswaran S, Ortega DR, Gralnick JA, El-Naggar MY and Jensen GJ. (2019) In situ imaging of the bacterial flagellar motor disassembly and assembly processes. EMBO J 38:e100957.

10. Shaffer CL*, Chang YW*, Rettberg L, Ghosal D, Jensen GJ. (2018) In vivo structures of the Helicobacter pylori Cag type IV secretion system. Cell Rep, 23(3): 673-681.

9. Ghosal D, Chang YW, Jeong KC, Vogel JP, Jensen GJ. (2017) In situ structure of the Legionella Dot/Icm type IV secretion system by electron cryotomography. EMBO Rep, 18, 726-732.

·        Reported in 1) Phys.Org 2) Caltech news 3) Futurity: Research News.

 8. Jeong KC, Ghosal D, Chang YW, Jensen GJ, Vogel JP. (2017) Polar delivery of Legionella type IV secretion system substrates is essential for virulence. PNAS, 114 (30) 8077-8082.

7. Yao Q, Jewett AI, Chang YW, Oikonomou CM, Beeby M, Iancu CV, Briegel A, Ghosal D, Jensen GJ. (2017) Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis. EMBO J, 1;36(11):1577-1589.

6. Briegel A, Oikonomou CM, Chang YW, Kjær A, Huang AN, Kim KW, Ghosal D, Nguyen HH, Kenny D, Ogorzalek Loo RR, Gunsalus RP, Jensen GJ. (2017) Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis. EMBO Rep, 18(9):1660-1670.

5. Girotra M, Srivastava S, Kulkarni A, Barbora A, Bobra K, Ghosal D, Devan P, Aher A, Jain A, Panda D, Ray K. (2017) The C-terminal tails of heterotrimeric kinesin-2 motor subunits directly bind to α-tubulin1: Possible implications for cilia-specific tubulin entry. Traffic, 18(2):123-133

4. Ghosal D †, Löwe J. (2015) Collaborative protein filaments. EMBO J, 14;34(18):2312-20.

3. Ghosal D, Trambaiolo D, Amos LA, Löwe J. (2014) MinCD cell division proteins form alternating copolymeric cytomotive filaments. Nature Communications. 15;5:5341.

• F1000 recommendation

2. Sadananda A, Hamid R, Doodhi H, Ghosal D, Girotra M, Jana SC, Ray K. (2012) Interaction with a kinesin-2 tail propels choline acetyltransferase flow towards synapse. Traffic. 13(7):979-91.

1. Doodhi H*, Ghosal D*, Krishnamurthy M, Jana SC, Shamala D, Bhaduri A, Sowdhamini R, Ray K. (2009) KAP, the accessory subunit of kinesin-2, binds the predicted coiled-coil stalk of the motor subunits. Biochemistry. 17;48(10):2248-60.

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Book chapters

1. Szwedziak P†, Ghosal D.† (2017) FtsZ-ring Architecture and Its Control by MinCD. Subcell Biochem. 84:213-244.

2. Ghosal D†, Löwe J, Amos L. A. (2016) Bacterial and Archaeal Cytoskeletons. Encyclopedia of Cell Biology. doi:10.1016/B978-0-12-394447-4.20058Mol Microbiol .